STUDY OF A CARDIAC ISCHEMIA MODEL INDUCED BY OXYGEN DEPRIVATION IN PHYSIOLOGICAL SOLUTION

Authors

DOI:

https://doi.org/10.14450/2318-9312.v37.e1.a2025.pp113-122

Keywords:

ischemia-reperfusion, superoxide dismutase, catalase, lipid peroxidation, cardiac damage

Abstract

This study aimed to assess the effect of the oxygen deprivation variant in inducing I/R injury in isolated rat hearts using histological and biochemical analyses. The hearts of the control group were perfused with oxygenated KH solution for 120 min, whereas the I/R group developed ischemia with KH solution without oxygen for 30 min. Our histological data provided evidence that the protocol used induced ischemia (Control= 6.20±1.43 vs I/R= 51.07±5.21% of the infarct area). Superoxide dismutase activity did not show significant differences between groups, while catalase exhibited a lower activity in the I/R group (37.56±4.67 U/mg protein) compared to the control group (55.37±4.13 U/mg protein). Lipid peroxidation was doubled in the I/R group (I/R= 4.21±0.49 nmol/mg; Control= 2.24±0.33 nmol/mg protein). A non-significant reduction of the mitochondrial complex I activity was shown in ischemic heart (Control= 1822±352.4 vs I/R= 1584±313.4 nmol.min-1. mg-1protein) and significant reduction in complex II (Control= 2326±156.6 vs I/R= 1512±245.8 nmol.min-1. mg-1 protein).

We suggest that the methodology described here is an excellent protocol, in which severe ischemia is associated with stress and mitochondrial damage.

Author Biographies

Xenia Hernández, University of Panama, Faculty of Medicine Maestría en Ciencias Biomédicas

Department of Biochemestry

Associate Professor

Ana Tejada, University of Panama, Faculty of Medicine

Department of Biochemestry

Full Professor

Juan Antonio Morán-Pinzón, University of Panama, Faculty of Medicine Centro de Investigaciones Psicofarmacológicas (CIPFAR)

Departmento of Pharmacology

Full Professor

Aldahir Mero Ríos, University of Panama, Faculty of Medicine Centro de Investigaciones Psicofarmacológicas (CIPFAR)

Department of Pharmacology

Associate Professor

Estela Ivonne Guerrero De León, University of Panama, Faculty of Medicine Centro de Investigaciones Psicofarmacológicas (CIPFAR)Sistema Nacional de Investigacion de Panama (SNI)

Departmento of Pharmacology

Full Professor

Downloads

Published

2026-03-31

How to Cite

Hernández, X., Tejada, A., Morán-Pinzón, J. A., Mero Ríos, A., & Guerrero De León, E. I. (2026). STUDY OF A CARDIAC ISCHEMIA MODEL INDUCED BY OXYGEN DEPRIVATION IN PHYSIOLOGICAL SOLUTION. Infarma - Pharmaceutical Sciences, 37(1), 113–122. https://doi.org/10.14450/2318-9312.v37.e1.a2025.pp113-122

Issue

Vol. 37 No. 1 (2025)

Section

Original article

License

Copyright (c) 2026 Xenia Hernández, Ana Tejada, Juan Antonio Morán-Pinzón, Aldahir Mero Ríos, Estela Ivonne Guerrero De León

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish in this journal agree to the following terms:

 

  1. Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Licença Creative Commons Attribution which allows the sharing of work with acknowledgment of authorship and initial publication in this journal.
  2. Authors are authorized to take additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (e.g. publish in institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.
  3. Authors are allowed and encouraged to publish and distribute their work online (e.g. in institutional repositories or on their personal page) at any point before or during the editorial process, as this can generate productive changes as well as increase the impact and Citation of published work (See O Efeito do Acesso Livre ).