NOVEL ELECTROCHEMICAL-BIOSENSORS FOR RAPID DETECTION OF SARS-COV-2

Authors

DOI:

https://doi.org/10.14450/2318-9312.v37.e1.a2025.pp23-29

Keywords:

SARS-CoV-2, COVID-19, sensores eletroquímicos

Abstract

This mini-review aimed to research the latest and most innovative platforms to detect SARS-CoV-2 and focused on electrochemical sensors based on virus-specific nucleic acid. The search for articles was performed using Web of Science, Scopus and Science Direct in the period of 2019-2022. The number of these researches decreased in 2022 due to the control of the pandemic, but the technologies involved in these developments have shown improvements in detection and possible application to other diseases.

Author Biography

Irene Satiko Kikuchi, Faculdade de Ciências Farmacêuticas da USP -Departamento de Farmácia - Av. Prof. Lineu Prestes, 580 - 05508-900 São Paulo – SP

Possui graduação em Farmácia e Bioquímica pela Faculdade de Ciências Farmacêuticas da Universidade de São Paulo Capital (1983), graduação em Tecnologia Em Processamento de Dados pelo Centro Estadual de Educação Tecnológica Paula Souza (1994) e doutorado em Ciências Biológicas (Bioquímica) pelo Instituto de Química da Universidade de São Paulo (2000). Pós-doutorado em Farmácia pela Faculdade de Ciências Farmacêuticas da USP (2003) e Drug Delivery System pela Universidade de Kyoto, Japão (2005/2006). Realizou treinamento e pesquisas em engenharia de microtecidos tridimensionais para biomateriais e medicina regenerativa na Yokohama National University, Japão (2016/2017). Atualmente é Professora Doutora na Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, Departamento de Farmácia. Atua em Controle de Qualidade de Medicamentos, Microbiologia de Microrganismos e na área de Bioquímica, com ênfase em Biologia Molecular e Celular, atuando principalmente nos seguintes temas: dna,nucleotídeo, câncer, lipossomos e antineoplásico, interações entre sistemas biológicos e não biológicos. Atuação voluntária como Membro Titular e atual Coordenadora do Comitê Técnico-Temático de Dispositivos Médicos da Farmacopéia Brasileira (ANVISA), tendo sido selecionada por edital desta Instituição.

References

Hatamluyi B, Rezayi M, Jamehdar SA, Rizi KS, Mojarrad M, Meshkat Z, Choobin H, Soleimanpour S, Boroushaki T. Sensitive and specific clinically diagnosis of SARS-CoV-2 employing a novel biosensor based on boron nitride quantum dots/flower-like gold nanostructures signal amplification. Biosens Bioelectron. 2022; 207: 114209. DOI: 10.1016/j.bios.2022.114209

Moher D, Liberati A, Tetzlaff J, Altman DG, Prisma Group. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009; 6(7): e1000097. DOI: 10.1371/journal.pmed.1000097

Ameku WA, Provance DW, Morel CM, De-Simone SG. Rapid detection of anti-SARS-CoV-2 antibodies with a secreen-printed electrode modified with a spike glycoprotein epitope. Biosensors. 2022;12:272. DOI: 10.3390/bios12050272

Cajigas S, Alzate D, Fernández M, Muskus C, Orozco J. Electrochemical genosensor for the specific detection of SARS-CoV-2. Talanta. 2022; 245:123482. DOI: 10.1016/j.talanta.2022.123482

Deng Y, Peng Y, Wang L, Wang M, Zhou T, Xiang L, Li J, Yang J. Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application. Anal Chim Acta. 2022; 1208: 339846. DOI:10.1016/j.aca.2022.339846

Farzin MA, Abdoos H, Saber R.Graphite nanocrystals coated paper-based electrode for detection of SARS-CoV-2 gene using DNA-functionalized Au@carbon dot core shell nanoparticles. Microchem J. 2022;179: 107585. DOI: 10.1016/j.microc.2022.107585

Haghayegh F, Salahandish R, Hassani M, Sanati-Nezhad A. Highly stable buffer-based zinc oxide/reduced graphene oxide nanosurface chemistry for rapid immunosensing of SARS-CoV-2 antigens. ACS Appl Mater Interfaces. 2022; 14: 10844-10855. DOI: 10.1021/acsami.1c24475

Hryniewicz BM, Volpe J, Bach-Toledo L, Kurpel KC, Deller AE, Soares AL, Nardin JM, Marchesi LF, Simas FF, Oliveira CC, Huergo L, Souto DEP, Vidotti M. Development of polypyrrole (nano)structures decorated with gold nanoparticles toward immunosensing for COVID-19 serological diagnosis. Mater Today Chem. 2022; 24: 100817. DOI: 10.1016/j.mtchem.2022.100817

Liu N, Liu R, Zhang J. CRISPR-Cas12a-mediated label-free electrochemical aptmer-based sensor for SARS-CoV-2 antigen detection. Bioelectrochemistry. 2022; 146: 108105. DOI: 10.1016/j.bioelechem.2022.108105

Nascimento ED, Fonseca WT, Oliveria TR, Correia CRSTB, Faça VM, Morais BP, Silvestrini VC, Pott-Junior H, Teixeira FR, Faria RC. COVID-19 diagnosis by SARS-CoV-2 Spike protein detection in saliva using an ultrasentive magneto-assay based on disposable electrochemical sensor. Sens Actuators B Chem. 2022; 353: 131128. DOI:10.1016/j.snb.2021.131128

Salahandish R, Jalali P, Tabrizi HO, Hyun JE, Haghayegh F, Khalghollah M, Zare A, Berenger BM, Niu YD, Ghafar-Zadeh E, Sanati-Nezhad A. A compact, low-cost, and binary sensing (BiSense) platform for noise-free and self-validated impedimetric detection of COVID-19 infected patients. Biosens Bioelectron. 2022; 213 114459. DOI:10.1016/j.bios.2022.114459

Wu C, Chen Z, Li C, Hao Y, Tang Y, Yuan Y, Chai L, Fan T, Yu J, Ma X, Al-Hartomy OA, Wageh S, Al-Sehemi AG, Luo Z, He Y, Li J, Xie Z, Zhang H. CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant. Nanomicro Lett. 2022; 14: 159. DOI:10.1007/s40820-022-00888-4

Wu L, Wang X, Wu C, Cao X, Tang T, Huang H, Huag X. Ultrasensitivite SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode. Anal Chim Acta. 2022; 1221: 340120. DOI:10.1016/j.aca.2022.340120

Yu M, Zhang X, Zhang X, Zahra Q, Huang, Z, Chen Y, Song C, Song M, Jiang H, Luo Z, Lu Y. An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19. Biosens Bioelectron. 2022; 213: 114436. DOI:10.1016/j.bios.2022.114436

Shi L, Wang L, Ma X, Fang X, Xiang L, Yi Y, Li H, Luo Z, Li G. Aptamer-functionalized nanochannels for one-step detection of SARS-CoV-2 in samples from COVID-19 patients. Anal Chem. 2021; 93(49):16646-16654. DOI: 10.1021/acs.analchem.1c04156

Downloads

Published

2026-03-31

How to Cite

Kikuchi, I. S., & Zuluaga, N. L. B. (2026). NOVEL ELECTROCHEMICAL-BIOSENSORS FOR RAPID DETECTION OF SARS-COV-2. Infarma - Pharmaceutical Sciences, 37(1), 23–29. https://doi.org/10.14450/2318-9312.v37.e1.a2025.pp23-29

Issue

Vol. 37 No. 1 (2025)

Section

Review Article

License

Copyright (c) 2026 Irene Satiko Kikuchi, Norma Lucía Buriticá Zuluaga, Irene Satiko Kikuchi

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish in this journal agree to the following terms:

 

  1. Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Licença Creative Commons Attribution which allows the sharing of work with acknowledgment of authorship and initial publication in this journal.
  2. Authors are authorized to take additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (e.g. publish in institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.
  3. Authors are allowed and encouraged to publish and distribute their work online (e.g. in institutional repositories or on their personal page) at any point before or during the editorial process, as this can generate productive changes as well as increase the impact and Citation of published work (See O Efeito do Acesso Livre ).